With the increase in pharmaceutical prescriptions for anxiety, this study may provide a safer and more natural option for those suffering from from this condition. In this study, a single application of hyprebaric oxygen therapy was shown to have an anxiolytic effect in mice. Typical HBOT programs involve the use of multiple sequential visits, in short duration. It would be therefore interesting to see if the conditioning of the brain through hyperbaric oxygen therapy can have a long-lasting effect on anxiety. Until further studies, we will not know, but what we do know is that there is an effect in the brain, probably mediated through the release of nitric oxide <view study>